Composite
Part:BBa_K4015016:Design
Designed by: Andrew Peng Yi Group: iGEM21_KEYSTONE (2021-10-21)
NusA-Lcp1VH2-HlyA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1371
Illegal XhoI site found at 2001
Illegal XhoI site found at 3526
Illegal XhoI site found at 3680 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1620
Illegal NgoMIV site found at 1978
Illegal NgoMIV site found at 3172
Illegal NgoMIV site found at 3494 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1605
Illegal BsaI.rc site found at 3075
Illegal SapI.rc site found at 1593
Design Notes
The NusA is for increasing solubility of Lcp, and the HlyA is for secretion.
Source
The parts are assembled through Gibson assembly, and the product is ligated to pET23a.