Composite

Part:BBa_K4015016:Design

Designed by: Andrew Peng Yi   Group: iGEM21_KEYSTONE   (2021-10-21)


NusA-Lcp1VH2-HlyA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1371
    Illegal XhoI site found at 2001
    Illegal XhoI site found at 3526
    Illegal XhoI site found at 3680
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1620
    Illegal NgoMIV site found at 1978
    Illegal NgoMIV site found at 3172
    Illegal NgoMIV site found at 3494
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1605
    Illegal BsaI.rc site found at 3075
    Illegal SapI.rc site found at 1593


Design Notes

The NusA is for increasing solubility of Lcp, and the HlyA is for secretion.


Source

The parts are assembled through Gibson assembly, and the product is ligated to pET23a.

References